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Literature summary for 3.4.22.15 extracted from
- An active 32-kDa cathepsin L is secreted directly from HT 1080 fibrosarcoma cells and not via lysosomal exocytosis (2015), PLoS ONE, 10, e0145067 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | thiol reagents are required for activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CLIK-148 | cathepsin L-specific inhibitor | Homo sapiens |  |
| CLIK-181 | cathepsin L-specific inhibitor | Homo sapiens | |
| E64 | inhibition of gelatinolytic activity | Homo sapiens | |
| leupeptin | inhibition of gelatinolytic activity and of autolcleavage of cathepsin L | Homo sapiens | |
| additional information | no inhibition by EDTA, PMSF, and pepstatin A | Homo sapiens | |
| N-acetyl-Leu-Leumethional | inhibition of gelatinolytic activity | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the 32 kDa form of cathepsin L is secreted directly from HT-1080 fibrosarcoma cells and not via lysosomal exocytosis, while cathepsin L is secreted via lysosomal exocytosis by several types of cancer cells, including prostate and breast cancer cells. Analysis of the mechanism underlying the secretion of the active 32-kDa cathepsin L form into the medium by HT 1080 cells, overview | Homo sapiens | - |
- |
| lysosome | - |
Homo sapiens | 5764 | - |
| additional information | cathepsin L is a lysosomal enzyme that is synthesized as a preproform and processed into a 41-kDa proform in the Golgi apparatus. The proform has two fates: to be targeted to lysosomes and to be secreted out of the cells | Homo sapiens | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P07711 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | investigation of the phosphorylation status of the secreted and lysosomal cathepsin L forms by treatment with alkaline phosphatase (ALP). The 32-kDa form of cathepsin L is phosphorylated, while the 34-kDa form is already dephosphorylated. The 32-kDa form might migrate to the anode faster than the dephosphorylated 34-kDa form because of the retention of the negative charge by the phosphate moiety. Acid phosphatase might remove the phosphorus group from the 32-kDa form as soon as it enters the lysosomes, thereby converting it to the 34-kDa form. The phosphorylated status of 32-kDa cathepsin L suggests that the secreted form has never enters lysosomes | Homo sapiens |
| proteolytic modification | cathepsin L is a lysosomal enzyme that is synthesized as a preproform and processed into a 41-kDa proform in the Golgi apparatus. An acidic pH of 4.8 is sufficient to initiate the autoactivation of single-chain cathepsin L intermediates into the mature double-chain form, this process is inhibited by leupeptin | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the enzyme binds to concanavalin A, but not to Lens culinaris agglutinin, Ricinus communis agglutinin 120 (RCA120), and wheat germ agglutinin | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| breast cancer cell | - |
Homo sapiens | - |
| carcinoma cell | - |
Homo sapiens | - |
| fibrosarcoma cell | - |
Homo sapiens | - |
| HT-1080 cell | - |
Homo sapiens | - |
| prostate gland cancer cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
| Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | the enzyme binds to concanavalin A, but not to Lens culinaris agglutinin, Ricinus communis agglutinin 120 (RCA120), and wheat germ agglutinin | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, extracellular form, SDS-PAGE, x * 34000, intracellular form, SDS-PAGE, x * 41000, pro-cathepsin L, SDS-PAGE | Homo sapiens |
| More | the active 32-kDa form is not derived from the extracellular processing of 41-kDa procathepsin L by surface activators. With leupeptin treatment, at least five bands are visible in zymography, two 32- and 34-kDa forms with strong activities and three 28.5-, 37-, and 39-kDa forms with faint activities | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cathepsin L | - |
Homo sapiens |
| cathepsin L1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
gelatinolytic activity | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | secreted 32 kDA cathepsin is 10times more active than intracellular 34 kDa cathepsin. Membrane trafficking of active 32-kDa cathepsin L, overview | Homo sapiens |
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